Non-league duo Sutton and Lincoln made history by both reaching the last 16, while Liverpool were the major casualties.
Here, Press Association Sport picks out five things we learned.
THE COMPETITION IS ALIVE
To pretend one exciting weekend means all is rosy in the FA Cup garden would be wrong, and the number of Premier League and Championship clubs fielding weakened teams was a major disappointment
But, when it comes to drama, the cup runneth over
Top billing has to go to Sutton and Lincoln, with the former writing another chapter in their remarkable story in the competition
Wolves stunned Liverpool at Anfield, while Fulham and Millwall also knocked out Premier League clubs
Wycombe, meanwhile, narrowly failed to topple Tottenham but their last-gasp 4-3 defeat was a cup classic.
IF AT FIRST YOU DON'T SUCCEED
Sutton first played in the fourth round 47 years ago when their opponents were Don Revie's all-conquering Leeds side
A record 14,000 people packed into Gander Green Lane to see United lose 6-0
In 1989 they were at it again, pulling off one of the most famous results in FA Cup history by beating Coventry
But the fourth round again proved beyond them, this time Norwich winning 8-0
Twenty-eight years later, by a quirk of FA Cup fate, Sutton found themselves drawn against Leeds once more after defeating AFC Wimbledon
And Jamie Collins' penalty won it for the non-leaguers.
DANNY WELBECK CAN BE IMPORTANT FOR ARSENAL
If any player deserves a break, it is Danny Welbeck
The striker has had an awful time with injuries since leaving Manchester United for Arsenal in the summer of 2014
Over the past two seasons, Welbeck has made just 19 appearances
Prior to being named in the starting line-up against Southampton on Saturday, he had played only a handful of minutes this season, but it did not show as he scored twice and put in an excellent performance for 65 minutes
If the 26-year-old can stay fit, he can offer both Arsenal and England a valuable option.
LIVERPOOL NEED SADIO MANE
It is no surprise that Liverpool have laid on a private jet to fly Sadio Mane back from the African Nations Cup following Senegal's exit on Saturday
In the forward's absence, the Reds have won just one of seven games - an FA Cup third-round replay against Plymouth - exited both domestic cup competitions and slipped 10 points behind Chelsea in the Premier League title race
Defence is an ongoing issue but Liverpool have also struggled to break teams down and have scored just five goals in those seven games
Mane's pace and penetration have been badly missed and, if he is available to face Chelsea on Tuesday, that will be a much-needed boost.
THE BIG GUNS ARE STILL FAVOURITES
For all the shocks, the chances of an outsider winning the competition still look remote
Only seven of the last 16 are Premier League teams - Leicester could join them after a replay against Derby - but Manchester United, Manchester City, Chelsea, Arsenal and Tottenham are all still in the hat
Since 1995, only Portsmouth and Wigan have broken the dominance of the big teams and the odds are stacked against the rest once again.
Source : PA