Hughes played on the left side of defence against the Shrimpers while Westlake operated as a left winger, and Wise is short of alternatives.
Jamie Clapham, who was signed on a short-term loan from Wolves this week, is a left-sided player and is poised to make his debut.
Ben Parker will also come into contention if both Westlake and Hughes are unavailable.
Sebastien Carole could return to the starting line-up after the Frenchman's startling performance as a substitute last Saturday.
Robert Bayly is available again after serving the three-match suspension he incurred on the final day of last season.
The Whites (from): Ankergren, Richardson, Heath, Marques, Hughes, Clapham, Gardner, Parker, Douglas, Thompson, Prutton, Carole, Weston, Howson, Bayly, Delph, Beckford, Kandol, Flo.