Dennis Wise may take the opportunity to rest a handful of senior players, although left-back Jamie Clapham is cup-tied after playing for Wolves in the first round of the competition.
Tom Elliott and Curtis Weston are believed to be carrying injuries while Tore Andre Flo is likely start the game on the bench, meaning Tresor Kandol and Jermaine Beckford leading the line again at Fratton Park.
The Whites (from): Ankergren, Richardson, Gardner, Heath, Marques, Parker, Hughes, Carole, Prutton, Howson, Douglas, Thompson, Bayly, Delph, Kandol, Beckford, Flo.