In front of a crowd of 2,180, the start was delayed by 15 minutes at Nethermoor, as Terry Dolan's players won with goals in either half from captain Richard Dunning and Robert Pell.
United's line-up was littered with reserve team players, and only one of their outfield players, last night's captain Robert Bayly, had a league start to their name.
The hosts produced the clearer chances during the opening half-hour, and both Mark Bett and James Hanson wasted early chances.
Tomi Ameobi and Nathan Cartman threatened at the other end, the latter forcing Guiseley keeper Steve Dickinson to tip his fierce shot over the crossbar, and Bayly, who stood out in midfield, also went close with a shot that drifted wide.
Guiseley opened the scoring in the 41st minute following a foul on the edge of United's box. Dunning lined up the free-kick and hooked his strike over a four-man wall and into the corner of Ankergren's net.
After the break, Cartman brought a save from Dickinson with a long-range shot in the 51st minute.
Tom Elliott also went close with a downward header which Dickinson smothered, but the match was settled by Pell's strike in the 57th minute.
The midfielder ran to the near post to meet a corner from Nathan Hay, and his header flicked across goal and beyond sub keeper Damion Ross.
United introduced a number of trialists but were unable to recover their two-goal deficit.
Guiseley: Dickinson, Hay, Wilford, Ellis, Ryan, Sturdy, Dunning, Krief, Hanson, Mumbley, Bett.
Subs: Rickers, Denton, Pell, Knight, Hall.
Leeds: Ankergren, Madden, Grace, Wilkinson, Fisher, Cartman, Bayly, Rothery, Delph, Ameobi, Elliott.
Subs: Hotchkiss, Ross, Ovington, Connor.
Attendance: 2,180.