Patrick Bamford could make his England debut on Sunday, just days after receiving his first international call-up.
However, Southgate insists sentiment will not play a role in his team selection for the World Cup qualifier as he prioritises the physical well being of his players ahead of next Wednesday's trip to Poland.
'It is not so much sentiment but we have a really strong squad and at this stage of the season players are still getting up to full fitness,' said Southgate.
'To be able to rotate and use the depth is very important. We have a group of players who deserve to play and any of them can go into the team and do a job as they have done when they have been in the past.
'Absolutely we will make a lot of changes, but what will be nice is that for those players who play on Sunday it will be the first game there (Wembley) since the Euros and they will get a fantastic reception I am sure.
'They deserve that hugely.' One player likely to be rested is Raheem Sterling, who produced yet another excellent performance in an England shirt, providing the opener before putting the team's second on a plate for Harry Kane.